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anti phlda2  (Proteintech)


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    Proteintech anti phlda2
    Anti Phlda2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+phlda2/PHLDA2+Antibody/pm40505801-79-8-10
    Average 93 stars, based on 10 article reviews
    anti phlda2 - by Bioz Stars, 2026-10
    93/100 stars

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    Article Title: LncRNA USP2-AS1 facilitates colorectal cancer development through the PHLDA2/PI3K/AKT axis.
    Article Snippet: Colorectal cancer (CRC) is one of the most common malignant tumors worldwide, seriously threatening human health.. Researchers have revealed that long non-coding RNAs (lncRNAs) are involved in the development of multiple cancers, including CRC.. In this study, we explored the expression level, roles, and mechanisms of lncRNA USP2-AS1 in CRC.

    Article Title: Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer
    Article Snippet: The following primary antibodies were used: Monoclonal anti-HA F-7 (Santa Cruz Biotechnology, sc-7392), anti-GST B-14 (sc-138), anti–Myc 9E10 (sc-40), anti-TFPI2 B-7 (sc-48380), anti-MEK1 H-8 (sc-6250), anti-HA 16B12 (Covance, MMS-101R), anti-HA 3F10 (Roche, 11867423001), anti-Flag M2 (Sigma–Aldrich, F1804), anti-phospho-Raf-1 56A6 (Cell Signaling Technology, 9427), anti-phospho-MEK1/2 41G9 (9154), anti-phospho-AKT1(T308) 244F9 (4056), anti-phospho-AKT1(S473) D9E (4060), anti-phospho-S6 D57.2.2E (4858), anti-S6 5G10 (2217), anti-MEK2 13E3 (9147), anti-GDF15 D2A3 (8479), anti-PHLDA1 EPR6674 (Abcam, ab133654), anti-PHLDA2 (ab58379), Anti-β-Actin 6D1 (FUJIFILM Wako, 010-27841); polyclonal anti-ERK (Santa Cruz Biotechnology, sc-94), anti-C-Raf-1 (sc-227), anti-B-Raf (sc-166), anti-phospho-ERK1/2 (Cell Signaling Technology, 9101), anti-AKT (9272), anti-cleaved caspase3 (9661), anti-PARP (9542), anti-COL14A1 (Abcam, ab101464), anti-TM4SF1 (ab113504), anti-PHLDA2 (Proteintech, 14661-1-AP), anti-TM4SF19 (Sigma-Aldrich, SAB1102826), anti-EMP1 (SAB1302714).

    Membrane:

    Article Title: Inhibition of PHLDA2 transcription by DNA methylation and YY1 in goat placenta.
    Article Snippet: Pleckstrin homology-like domain family A member 2 (PHLDA2) is essential for placental development in mammals.. This study was conducted to investigate transcriptional regulation of goat PHLDA2 in the placenta.. Real-time PCR and Western blot analyses showed different expression of the PHLDA2 in goat placentas during gestation with highest expression at 30 and 45 days post coitus (P < 0.05).



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    Identification of difference-related genes affected by SNDPC in HCC. (A) Heatmaps, (B) volcano plot, (C,D) GO, (E,F) pathway, and (G,H) Venn were used to analyze the difference-related genes in HCC. (I) Western blotting was used to determine the expression of <t>PHLDA2</t> and SPINK1. The results are presented as the mean ± SD. ***, P<0.001. GO, Gene Ontology; TCGA, The Cancer Genome Atlas; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; SNDPC, Sini decoction-polysaccharide compound; HCC, hepatocellular carcinoma; SD, standard deviation.
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    Identification of difference-related genes affected by SNDPC in HCC. (A) Heatmaps, (B) volcano plot, (C,D) GO, (E,F) pathway, and (G,H) Venn were used to analyze the difference-related genes in HCC. (I) Western blotting was used to determine the expression of <t>PHLDA2</t> and SPINK1. The results are presented as the mean ± SD. ***, P<0.001. GO, Gene Ontology; TCGA, The Cancer Genome Atlas; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; SNDPC, Sini decoction-polysaccharide compound; HCC, hepatocellular carcinoma; SD, standard deviation.
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    Identification of difference-related genes affected by SNDPC in HCC. (A) Heatmaps, (B) volcano plot, (C,D) GO, (E,F) pathway, and (G,H) Venn were used to analyze the difference-related genes in HCC. (I) Western blotting was used to determine the expression of <t>PHLDA2</t> and SPINK1. The results are presented as the mean ± SD. ***, P<0.001. GO, Gene Ontology; TCGA, The Cancer Genome Atlas; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; SNDPC, Sini decoction-polysaccharide compound; HCC, hepatocellular carcinoma; SD, standard deviation.
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    antibodies against phlda2  (Proteintech)
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    Figure 11. Validation of <t>PHLDA2</t> expression in HCC. (A) Expression levels of PHLDA2 in HCC tissues and adjacent tissues by IHC. (B) Immunohistochemical analysis of PHLDA2 expression in 58 pairs of HCC tissues and adjacent tissues. (C) Western Blot analysis of PHLDA2 expression in 6 pairs of HCC tissues and adjacent tissues.
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    Figure 11. Validation of <t>PHLDA2</t> expression in HCC. (A) Expression levels of PHLDA2 in HCC tissues and adjacent tissues by IHC. (B) Immunohistochemical analysis of PHLDA2 expression in 58 pairs of HCC tissues and adjacent tissues. (C) Western Blot analysis of PHLDA2 expression in 6 pairs of HCC tissues and adjacent tissues.
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    Identification of difference-related genes affected by SNDPC in HCC. (A) Heatmaps, (B) volcano plot, (C,D) GO, (E,F) pathway, and (G,H) Venn were used to analyze the difference-related genes in HCC. (I) Western blotting was used to determine the expression of PHLDA2 and SPINK1. The results are presented as the mean ± SD. ***, P<0.001. GO, Gene Ontology; TCGA, The Cancer Genome Atlas; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; SNDPC, Sini decoction-polysaccharide compound; HCC, hepatocellular carcinoma; SD, standard deviation.

    Journal: Translational Cancer Research

    Article Title: Sini decoction-polysaccharide compound regulates proliferation, apoptosis, and glycolysis of liver cancer cells through PHLDA2/ANXA2

    doi: 10.21037/tcr-24-1625

    Figure Lengend Snippet: Identification of difference-related genes affected by SNDPC in HCC. (A) Heatmaps, (B) volcano plot, (C,D) GO, (E,F) pathway, and (G,H) Venn were used to analyze the difference-related genes in HCC. (I) Western blotting was used to determine the expression of PHLDA2 and SPINK1. The results are presented as the mean ± SD. ***, P<0.001. GO, Gene Ontology; TCGA, The Cancer Genome Atlas; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; SNDPC, Sini decoction-polysaccharide compound; HCC, hepatocellular carcinoma; SD, standard deviation.

    Article Snippet: After the membranes were incubated overnight, they were sealed with 5% bovine serum albumin for 2 h and with primary antibodies against Bcl-2 (ab196495; Abcam, Cambridge, UK), Bax (ab32503; Abcam), cleaved caspase 3 (25128-1-AP; Proteintech, Rosemont, IL, USA), caspase 3 (82202-1-RR; Proteintech), LDHA [#2012; Cell Signaling Technology (CST), Danvers, MA, USA], HK2 (22029-1-AP; Proteintech), PKM2 (#3198; CST), PHLDA2 (14661-1-AP; Proteintech), SPINK1 (13477-1-AP; Proteintech), ANXA2 (#8235; CST), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; #2118; CST) at 4 °C.

    Techniques: Western Blot, Expressing, Standard Deviation

    SNDPC regulated the expression of ANXA2 via PHLDA2. The (A) MEM database, (B) HCCDB, (C) LinkedOmics database, and (D) GEPIA database were used to determine the correlation between PHLDA2 and ANXA2. (E) ANXA2 protein level after treatment with 10–40 mg/mL if SNDPC. (F) A co-IP assay was used to confirm the combination of PHLDA2 and ANXA2. (G) The protein concentration of PHLDA2 after PHLDA2 overexpression was detected using Western blotting. (H) The protein concentration of PHLDA2 in HCCLM3 cells with PHLDA2 overexpression and SNDPC. The results are presented as the mean ± SD. ***, P<0.001; ### , P<0.001. TPM, transcripts per million; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Ov-NC, empty vector; Ov-PHLDA2, PHLDA2-specific pcDNA-overexpression vector; pcDNA, protruding clustered DNA; SNDPC, Sini decoction-polysaccharide compound; MEM, Multi-Experiment Matrix; GEPIA, Gene Expression Profiling Interactive Analysis; co-IP, coimmunoprecipitation; SD, standard deviation.

    Journal: Translational Cancer Research

    Article Title: Sini decoction-polysaccharide compound regulates proliferation, apoptosis, and glycolysis of liver cancer cells through PHLDA2/ANXA2

    doi: 10.21037/tcr-24-1625

    Figure Lengend Snippet: SNDPC regulated the expression of ANXA2 via PHLDA2. The (A) MEM database, (B) HCCDB, (C) LinkedOmics database, and (D) GEPIA database were used to determine the correlation between PHLDA2 and ANXA2. (E) ANXA2 protein level after treatment with 10–40 mg/mL if SNDPC. (F) A co-IP assay was used to confirm the combination of PHLDA2 and ANXA2. (G) The protein concentration of PHLDA2 after PHLDA2 overexpression was detected using Western blotting. (H) The protein concentration of PHLDA2 in HCCLM3 cells with PHLDA2 overexpression and SNDPC. The results are presented as the mean ± SD. ***, P<0.001; ### , P<0.001. TPM, transcripts per million; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Ov-NC, empty vector; Ov-PHLDA2, PHLDA2-specific pcDNA-overexpression vector; pcDNA, protruding clustered DNA; SNDPC, Sini decoction-polysaccharide compound; MEM, Multi-Experiment Matrix; GEPIA, Gene Expression Profiling Interactive Analysis; co-IP, coimmunoprecipitation; SD, standard deviation.

    Article Snippet: After the membranes were incubated overnight, they were sealed with 5% bovine serum albumin for 2 h and with primary antibodies against Bcl-2 (ab196495; Abcam, Cambridge, UK), Bax (ab32503; Abcam), cleaved caspase 3 (25128-1-AP; Proteintech, Rosemont, IL, USA), caspase 3 (82202-1-RR; Proteintech), LDHA [#2012; Cell Signaling Technology (CST), Danvers, MA, USA], HK2 (22029-1-AP; Proteintech), PKM2 (#3198; CST), PHLDA2 (14661-1-AP; Proteintech), SPINK1 (13477-1-AP; Proteintech), ANXA2 (#8235; CST), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; #2118; CST) at 4 °C.

    Techniques: Expressing, Co-Immunoprecipitation Assay, Protein Concentration, Over Expression, Western Blot, Plasmid Preparation, Gene Expression, Standard Deviation

    PHLDA2 deficiency suppressed HCCLM3 cell proliferation ability, reduced glycolysis, and facilitated cell apoptosis. (A) The mRNA expression and (B) protein level of PHLDA2 in HCCLM3 cells were determined using qRT-PCR and Western blotting. (C) CCK-8 assay was used to determine cell proliferation. (D,E) The level of cell apoptosis was determined using TUNEL assay. (F) Western blotting was used to estimate apoptosis-related protein levels. (G) Glucose uptake, (H) lactate concentration, and (I) ECAR were detected after PHLDA2 silencing. (J) The protein levels of LDHA, HK2, and PKM2 in HCCLM3 cells were determined using Western blotting. The results are presented as the mean ± SD. ***, P<0.001. siRNA-NC, corresponding control siRNA; siRNA, small interfering RNA; siRNA-PHLDA2-1/2, specific siRNA against PHLDA2; mRNA, messenger RNA; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling; DAPI, 4',6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ECAR, extracellular acidification rate; qRT-PCR, quantitative real-time polymerase chain reaction; CCK-8, Cell Counting Kit-8; SD, standard deviation.

    Journal: Translational Cancer Research

    Article Title: Sini decoction-polysaccharide compound regulates proliferation, apoptosis, and glycolysis of liver cancer cells through PHLDA2/ANXA2

    doi: 10.21037/tcr-24-1625

    Figure Lengend Snippet: PHLDA2 deficiency suppressed HCCLM3 cell proliferation ability, reduced glycolysis, and facilitated cell apoptosis. (A) The mRNA expression and (B) protein level of PHLDA2 in HCCLM3 cells were determined using qRT-PCR and Western blotting. (C) CCK-8 assay was used to determine cell proliferation. (D,E) The level of cell apoptosis was determined using TUNEL assay. (F) Western blotting was used to estimate apoptosis-related protein levels. (G) Glucose uptake, (H) lactate concentration, and (I) ECAR were detected after PHLDA2 silencing. (J) The protein levels of LDHA, HK2, and PKM2 in HCCLM3 cells were determined using Western blotting. The results are presented as the mean ± SD. ***, P<0.001. siRNA-NC, corresponding control siRNA; siRNA, small interfering RNA; siRNA-PHLDA2-1/2, specific siRNA against PHLDA2; mRNA, messenger RNA; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling; DAPI, 4',6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ECAR, extracellular acidification rate; qRT-PCR, quantitative real-time polymerase chain reaction; CCK-8, Cell Counting Kit-8; SD, standard deviation.

    Article Snippet: After the membranes were incubated overnight, they were sealed with 5% bovine serum albumin for 2 h and with primary antibodies against Bcl-2 (ab196495; Abcam, Cambridge, UK), Bax (ab32503; Abcam), cleaved caspase 3 (25128-1-AP; Proteintech, Rosemont, IL, USA), caspase 3 (82202-1-RR; Proteintech), LDHA [#2012; Cell Signaling Technology (CST), Danvers, MA, USA], HK2 (22029-1-AP; Proteintech), PKM2 (#3198; CST), PHLDA2 (14661-1-AP; Proteintech), SPINK1 (13477-1-AP; Proteintech), ANXA2 (#8235; CST), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; #2118; CST) at 4 °C.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay, TUNEL Assay, Concentration Assay, Control, Small Interfering RNA, Real-time Polymerase Chain Reaction, Cell Counting, Standard Deviation

    SNDPC suppressed HCC cell proliferation and glycolysis and enhanced apoptosis via PHLDA2/ANXA2. (A) CCK-8 assay was used to assess cell proliferation ability. (B,C) The level of cell apoptosis was determined using TUNEL assay. (D) Western blotting was used to determine the apoptosis-related proteins. (E) Glucose uptake, (F) lactate concentration, (G) and the ECAR were detected after PHLDA2 overexpression. (H) The protein levels of LDHA, HK2, and PKM2 in HCCLM3 cells were measured using Western blotting. The results are presented as the mean ± SD. ***, P<0.001; ### , P<0.001. SNDPC, Sini decoction-polysaccharide compound; Ov-NC, empty vector; Ov-PHLDA2, PHLDA2-specific pcDNA-overexpression vector; pcDNA, protruding clustered DNA; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling; DAPI, 4',6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HCC, hepatocellular carcinoma; CCK-8, Cell Counting Kit-8; ECAR, extracellular acidification rate; SD, standard deviation.

    Journal: Translational Cancer Research

    Article Title: Sini decoction-polysaccharide compound regulates proliferation, apoptosis, and glycolysis of liver cancer cells through PHLDA2/ANXA2

    doi: 10.21037/tcr-24-1625

    Figure Lengend Snippet: SNDPC suppressed HCC cell proliferation and glycolysis and enhanced apoptosis via PHLDA2/ANXA2. (A) CCK-8 assay was used to assess cell proliferation ability. (B,C) The level of cell apoptosis was determined using TUNEL assay. (D) Western blotting was used to determine the apoptosis-related proteins. (E) Glucose uptake, (F) lactate concentration, (G) and the ECAR were detected after PHLDA2 overexpression. (H) The protein levels of LDHA, HK2, and PKM2 in HCCLM3 cells were measured using Western blotting. The results are presented as the mean ± SD. ***, P<0.001; ### , P<0.001. SNDPC, Sini decoction-polysaccharide compound; Ov-NC, empty vector; Ov-PHLDA2, PHLDA2-specific pcDNA-overexpression vector; pcDNA, protruding clustered DNA; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling; DAPI, 4',6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HCC, hepatocellular carcinoma; CCK-8, Cell Counting Kit-8; ECAR, extracellular acidification rate; SD, standard deviation.

    Article Snippet: After the membranes were incubated overnight, they were sealed with 5% bovine serum albumin for 2 h and with primary antibodies against Bcl-2 (ab196495; Abcam, Cambridge, UK), Bax (ab32503; Abcam), cleaved caspase 3 (25128-1-AP; Proteintech, Rosemont, IL, USA), caspase 3 (82202-1-RR; Proteintech), LDHA [#2012; Cell Signaling Technology (CST), Danvers, MA, USA], HK2 (22029-1-AP; Proteintech), PKM2 (#3198; CST), PHLDA2 (14661-1-AP; Proteintech), SPINK1 (13477-1-AP; Proteintech), ANXA2 (#8235; CST), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; #2118; CST) at 4 °C.

    Techniques: CCK-8 Assay, TUNEL Assay, Western Blot, Concentration Assay, Over Expression, Plasmid Preparation, Cell Counting, Standard Deviation

    Figure 11. Validation of PHLDA2 expression in HCC. (A) Expression levels of PHLDA2 in HCC tissues and adjacent tissues by IHC. (B) Immunohistochemical analysis of PHLDA2 expression in 58 pairs of HCC tissues and adjacent tissues. (C) Western Blot analysis of PHLDA2 expression in 6 pairs of HCC tissues and adjacent tissues.

    Journal: Scientific reports

    Article Title: Characteristics of lactate metabolism phenotype in hepatocellular carcinoma.

    doi: 10.1038/s41598-023-47065-0

    Figure Lengend Snippet: Figure 11. Validation of PHLDA2 expression in HCC. (A) Expression levels of PHLDA2 in HCC tissues and adjacent tissues by IHC. (B) Immunohistochemical analysis of PHLDA2 expression in 58 pairs of HCC tissues and adjacent tissues. (C) Western Blot analysis of PHLDA2 expression in 6 pairs of HCC tissues and adjacent tissues.

    Article Snippet: IHC was conducted using antibodies against PHLDA2 from Proteintech Group Inc. (14661-1-AP, 1:1000, Chicago, USA).

    Techniques: Biomarker Discovery, Expressing, Immunohistochemical staining, Western Blot